RPPA

Reverse-phase protein array (RPPA) is a high-throughput technology that performs protein assays on thousands of samples simultaneously that measure the levels of protein expression, as well as protein modifications such as phosphorylation. Protein lysates are arrayed as microspots on nitrocellulized coated glass slides and probed with highly specific antibodies that have been validated for RPPA. When specific antibodies are used, RPPA can generate 1,000 times more data points using 10,000 times less sample volume than an ordinary western blot, enabling researchers to monitor quantitative proteomic responses for various time-scale and input-dose gradients simultaneously.